Journal: Histochemistry and Cell Biology
Article Title: Stage-specific expression of Toll-like receptors in the seminiferous epithelium of mouse testis
doi: 10.1007/s00418-024-02310-z
Figure Lengend Snippet: Expression pattern of TLR-3 in the cycle of seminiferous epithelium. Anti-TLR-3 (NB100-56571, Novus, 1/50) primary antibody was used for detection of cells expressing TLR-3. Immune-positive cells expressing TLR-3 ( a , b , c ) appear brown in color. TLR-3 is expressed in spermatocytes, round and elongated spermatids, endosomal compartments, and acrosomes ( a , b , c ). Please note that PAS staining on the corresponding sequential section (Supplementary Fig. ) indicates that TLR-3 is expressed at early, middle, and late stages of spermatogenesis. Arrow heads indicate immunopositivity at the acrosome. eSt elongated spermatid, Ec endosomal compartment, rSt round spermatid, *spermatocytes, and arrows indicate spermatogonia
Article Snippet: Anti-TLR-1 (B-23, Sc-130896, Santa Cruz Biotechnology, 1/50), anti-TLR 2 (NB100-56720, Novus, 1/50), anti-TLR-3 (NB100-56571, Novus, 1/50), anti-TLR-4 (NB100- 56,566, Novus, 1/50), anti-TLR-5 (H-127, Sc-10742, Santa Cruz Biotechnology, 1/50), anti-TLR-6 (NBP1-54,336, Novus, 1/50), anti-TLR-7 (NB100-56682, Novus, 1/50), anti-TLR-8 (NBP2- 24,917, Novus, 1/50), anti-TLR-9 (NBP2-24,729, Novus, 1/50), anti-TLR-11 (NBP1-77,204, Novus, 1/50), anti-TLR-12 (NBP2-24,833, Novus, 1/50), and anti-TLR-13 (NBP2-24,539, Novus, 1/50) were used as primary antibodies.
Techniques: Expressing, Staining